b'V A S H EW O U N D S O L U T I O NUse with Negative Pressure Disruption of Biofilm (in Vitro)Wound Therapy:Additional studies have suggested the use of Vashe as an MINIMIZE RISK THROUGH BIOFILMinstillation solution with Negative Pressure Wound Therapy DISRUPTION AND REMOVAL OFled to faster granulation tissue formation and healthier wound CAUSATIVE PATHOGENS beds. 29Biofilm, the exopolymeric substance secreted by and surrounding bacteria, has presented many problems in the Negative Wound Pressure Therapy is one of the most documentedhealing of wounds. Estimates suggest biofilm is present and wound therapies in the world. 25Recently, the instillation applicationpotentially delaying healing in 60% of chronic wounds. 31was added to the portfolio of these products. Vashe is effective atThe ability of Vashe to disrupt biofilms has been studied disrupting biofilms and removing debris and microorganisms fromextensively. Laboratory studies have shown that hypochlorous wounds, resulting in faster healing, fewer OR visits, and earlieracid has the ability to disrupt 90% of biofilms after just a short discharges when compared to saline and Dakins solution. 26-28 order of exposure. 26Disrupting biofilm is a crucial element of any wound care clinicians armamentarium in treating chronic wounds.Patients who received irrigation with hypochlorous acid solutionshowed improved removal of devitalized tissue and improved tissue granulation compared to those who received saline29 Effect of Hypochlorous Acid on Biofilmwhen the rest of their treatment regimen was kept the same. S. aureus Bacterial NumbersAn observational study showed that using Vashe WoundSolution with NPWT may decrease the length of hospital stays,7operating room visits, and days until wound closure. 30 ) S. aureus (CFU/cm 62Using NPWT therapy such as VERAFLO Therapy with Instillation5 coupled with Vashe Wound Solution has been shown to4remove nonviable tissue and debris and to shorten wound bed3preparation compared to saline. 27 210Mean 0 1 3 5 7 10Solution Used Operating RoomLength of Stay Days to Contact Time (minutes)Visits ClosureNaOCl/NaCl 7 25 37 Effect of Hypochlorous Acid on Polysaccharide Vashe 3.2 14 30 Levels Within S. aureus Biofilm Matrix10) Polysaccharide (ug/cm28Use of Vashe in Irrigation: 6LEAVE NOTHING TO CHANCE4GET BACTERIAL COUNTS DOWN AND2KEEP THEM DOWN 0 0 1 3 5 7 10Vashe Wound Solution has demonstrated superior outcomesContact Time (minutes)related to flap closure when compared to saline. This study used ultrasonic debridement systems 7 days prior to closure,Effect of Hypochlorous Acid on Protein Levelsand bacteria counts were measured through quantitativewithin S. aureus Biofilm Matrixbacteriology. In wounds treated with Vashe, bacteria counts not only diminished, but stayed low for up to 7 days, resulting in2550% greater closure than saline-treated wounds. 22 ) Protein (ug/cm 20215UltrasonicInitial Post-7 days Failure 10DebridementBacteria DebridementPost-DebridementRateSolution Count Count Count of Flap 5NaCl .9% 10 6 10 2 10 5 80% 00 1 3 5 7 10Vashe 10 6 10 2 10 2 25% Contact Time (minutes)'